Introduction
CD163isamembraneproteinandmemberofthegroupBScavengerReceptorCysteine-Richsuperfamilyspecificallyexpressedonperipheralbloodmonocytesandmacrophages.Aparticularlyhighexpressionisseeninmacrophagesofthe‘alternativeactivation’phenotypeplayingamajorroleindampeningtheinflammatoryresponseandinscavengingcomponentsofdamagedcells.CD163functionsasthereceptorforHaptoglobin-hemoglobincomplexes,andfurThermoreCD163isinvolvedintheregulationofinflammation.
Macrophagesplayacentralroleinthehostresponsetoinfectionandtissuedamage,andarefurthermoreimportantinthepathogenesisofautoimmunediseasesandcancer.
MeasurementofsCD163maybeavaluableMarkerindiseaseswithmacrophageand/ormonocyteinvolvement,suchasmacrophageactivationsyndromes(e.g.hemophagocyticsyndrome),infections,liverdisease,auto-immunedisease,atherosclerosisandcancer.Additionally,CD163positivemacrophagesconstituteamayorcellsubpopulationinhumantermplacentasuggestingarolefortheplacentafunctioningasanactiveimmunosuppressiveBIOLOGicalbarrierbetweenmotherandfetus.
PrincipleofMacro163™
TheassayisbasedontheprincipleofthesandwichELISA.ApolyclonalantibodyrecognizingCD163isimmobilizedonthesurfaceofamicrotiterplate.
AfterincubationwiththesampleorrecombinantCD163standardasecondbiotinylatedmonoclonalantibodyrecognizingCD163isadded.Detectionofthelatterisdonebyaddingstreptavidin-HRP.UsingTMB(3,3’,5,5’-tetramethylbenzidine)assubstratefortheenzymeHRP,theamountofsCD163proteincanbequantified.
Intendeduse
- ThisMacro163™kitisintendedforthequantificationofsolubleCD163(sCD163)inserumorplasmasamples.
- TheMacro163™assayhasbeenvalidatedforserumandplasmameasurementsbutcanalsobeappliedtootherbiologicalfluidslikesynovialfluid,ascitesfluid,pleuralfluid,cerebrospinalfluidandcell-supernatants.
- Designedforresearchpurposesonly.